| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ramgulam, Varshana (11)
|
ADCPP |
38.47 |
36.84 |
-1.63 |
| 2 |
Jurgelon, Amanda M (12)
|
ADGUY |
|
37.78 |
- |
| 3 |
Simmons, Riley C (12)
|
ADCPP |
36.99 |
39.11 |
2.12 |
| 4 |
Zhang, Stella Y (11)
|
ADCPP |
38.66 |
39.14 |
0.48 |
| 5 |
McCoy, Sophia H (12)
|
ADCPP |
41.38 |
39.77 |
-1.61 |
| 6 |
Gayle, Isabella C (12)
|
ADCPP |
39.84 |
39.78 |
-0.06 |
| 7 |
Boenau, Carlye Ana T (11)
|
ADBFIN |
39.06 |
39.83 |
0.77 |
| 8 |
Napier, Clio A (12)
|
ADUN |
|
40.33 |
- |
| 9 |
Michels, Delaney R (11)
|
ADCPP |
37.42 |
40.38 |
2.96 |
| 10 |
Bieniek, Allison M (11)
|
ADCPP |
39.44 |
40.51 |
1.07 |
| 11 |
Stoup, Anna L (11)
|
ADCPP |
38.75 |
41.30 |
2.55 |
| 12 |
Pazderski, Carlie M (12)
|
ADCPP |
41.88 |
41.72 |
-0.16 |
| 13 |
Carsillo, Gianna I (12)
|
ADDELM |
41.46 |
42.15 |
0.69 |
| 14 |
Xue, Nichole Y (11)
|
ADCPP |
42.18 |
42.26 |
0.08 |
| 15 |
Conklin, Charlotte E (12)
|
ADCPP |
46.68 |
45.29 |
-1.39 |
| 16 |
Joshi, Aishi (11)
|
ADCPP |
49.21 |
51.44 |
2.23 |
| 17 |
Leonard, Emma G (12)
|
ADDELM |
50.21 |
52.70 |
2.49 |
| 18 |
Chen, RuiXuan (11)
|
ADCPP |
51.54 |
53.33 |
1.79 |
| 19 |
Franco, Madilynn R (11)
|
ADCPP |
51.88 |
53.69 |
1.81 |
| 20 |
Giebel, Catherine E (11)
|
ADCPP |
51.64 |
57.01 |
5.37 |
|
Sands, Samantha B (11)
|
ADCPP |
41.07 |
NS |
- |
|
Greaux, Lauren A (11)
|
ADDELM |
40.74 |
NS |
- |